四、結論

在(zai)(zai)(zai)這項工作中，我們研究了 16-E2-16 與(yu)(yu) 通過使(shi)用(yong)(yong)紫外-可見光(guang)(guang)、熒光(guang)(guang)和(he) CD 光(guang)(guang)譜(pu)技(ji)術結(jie)合(he)計(ji)算(suan)方(fang)法來計(ji)算(suan) Hb。 此(ci)外， 該相互作用(yong)(yong)系統(tong)也采用(yong)(yong)表(biao)面(mian)張(zhang)力(li)法。 在(zai)(zai)(zai) UV-vis 研究的(de)(de)(de)(de)(de)幫助下，我們發現 16-E2-16 導(dao)致(zhi)血紅素(su)基團暴露于(yu)水性介質(zhi)。 熒光(guang)(guang)光(guang)(guang)譜(pu)證實內在(zai)(zai)(zai)猝(cu)滅 16-E2-16 的(de)(de)(de)(de)(de)滲透抑(yi)制了血紅素(su)對(dui) Trp 的(de)(de)(de)(de)(de)影響 進入 Hb 的(de)(de)(de)(de)(de)疏水腔并導(dao)致(zhi)熒光(guang)(guang)強度增加。 效果還是比(bi)較明顯的(de)(de)(de)(de)(de) 只到cmc。 Trp 熒光(guang)(guang)比(bi) Tyr 受到的(de)(de)(de)(de)(de)影響更(geng)大 如同步光(guang)(guang)譜(pu)所示。 表(biao)面(mian)張(zhang)力(li)結(jie)果 確(que)定 16-E2-16 的(de)(de)(de)(de)(de) cmc 值在(zai)(zai)(zai)存在(zai)(zai)(zai)的(de)(de)(de)(de)(de)情況下增加 Hb 以及隨著溫(wen)度升高(gao)而 cac 保持不變 在(zai)(zai)(zai)所有溫(wen)度下保持恒定。 表(biao)面(mian)張(zhang)力(li)測量也 表(biao)明膠束化過程是自(zi)發的(de)(de)(de)(de)(de)、放熱的(de)(de)(de)(de)(de)和(he) 熵驅動。 此(ci)外，得出結(jie)論，吸附在(zai)(zai)(zai) 界(jie)面(mian)優于(yu)膠束化。 分子建模方(fang)法表(biao)明 16-E2-16 在(zai)(zai)(zai) Hb 中的(de)(de)(de)(de)(de)結(jie)合(he) 位于(yu)α1和(he)β2鏈(lian)中。 它還提(ti)供了對(dui)我們的(de)(de)(de)(de)(de)驗(yan)證 通過確(que)認主要結(jie)合(he)力(li)的(de)(de)(de)(de)(de)實驗(yan)結(jie)果 是疏水的(de)(de)(de)(de)(de)和(he)靜電的(de)(de)(de)(de)(de)。 圓二色性表(biao)明 16-E2-16 與(yu)(yu) Hb 結(jie)合(he)導(dao)致(zhi) α-螺旋含(han)量減少。 MD 模擬結(jie)果表(biao)明 Hb 和(he) 16-E2-16 結(jie)合(he) Hb 穩定在(zai)(zai)(zai) 6000 ps 左右并確(que)認構象 與(yu)(yu) 16-E2-16 相互作用(yong)(yong)后 Hb 發生了變化，如 我們的(de)(de)(de)(de)(de) CD 結(jie)果顯示。

致謝

Rajan Patel 博士(shi)非常感(gan)謝(xie)來自 科學(xue)(xue)與工(gong)程研(yan)究(jiu)委員(yuan)會（SR/S1/PC-19/2011 和(he) SB/EMEQ-097/2013) 和(he)新德里大(da)學(xue)(xue)撥款(kuan)委員(yuan)會， 印度(du)（F.No. 39-841/2010 (SR)）。 Abbul Bashar Khan 博士(shi)表(biao)示感(gan)謝(xie) 印度(du)新德里科學(xue)(xue)與工(gong)程研(yan)究(jiu)委員(yuan)會， 提(ti)供研(yan)究(jiu)資(zi)助 (SB/FT/CS-031/2013)。 作者還 感(gan)謝(xie) DST 為 FIST 撥款(kuan)提(ti)供制裁令編號。 （SR/FIST/LS-541/2012）。

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酯功能化的雙子表面活性劑與血紅蛋白的結合——摘要、簡介

酯功能化的雙子表面活性劑與血紅蛋白的結合——材料和方法

酯功能化的雙子表面活性劑與血紅蛋白的結合——結果和討論

酯功能化的雙子表面活性劑與血紅蛋白的結合——結論、致謝！