結論

本(ben)(ben)(ben)文報道的(de)(de)(de)(de)(de)數據表(biao)(biao)(biao)(biao)(biao)(biao)明(ming)，固定(ding)(ding)在(zai)(zai)DMPA單分(fen)(fen)子(zi)膜上的(de)(de)(de)(de)(de)DSAP的(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)活(huo)(huo)性(xing)(xing)受表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)密(mi)度和(he)表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)彈性(xing)(xing)的(de)(de)(de)(de)(de)調節(jie)，兩者均受單分(fen)(fen)子(zi)膜表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)堆積(ji)(ji)(ji)的(de)(de)(de)(de)(de)影響(xiang)。 在(zai)(zai)高(gao)壓(ya)縮(suo)性(xing)(xing)條件下(xia)，影響(xiang)酶(mei)(mei)(mei)(mei)(mei)活(huo)(huo)性(xing)(xing)的(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)(yao)因素(su)是表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)密(mi)度，而最大(da)值來(lai)(lai)自表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)密(mi)度和(he)最佳(jia)(jia)膜彈性(xing)(xing)的(de)(de)(de)(de)(de)組(zu)合(he)，取(qu)決(jue)于脂(zhi)(zhi)(zhi)(zhi)質(zhi)/DSAP表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)填(tian)充(chong)。 在(zai)(zai)高(gao)表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)壓(ya)力(li)(li)(li)下(xia)，導致多肽部(bu)分(fen)(fen)聚集和(he)界面(mian)(mian)(mian)(mian)(mian)(mian)流(liu)動(dong)性(xing)(xing)降(jiang)低（如壓(ya)縮(suo)模(mo)量顯著增(zeng)加所(suo)(suo)示(shi)(shi)）的(de)(de)(de)(de)(de)分(fen)(fen)子(zi)間相互(hu)(hu)作用可(ke)(ke)能與(yu)阻礙水溶性(xing)(xing)底(di)(di)物(wu)進入(ru)酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)催(cui)(cui)化(hua)(hua)活(huo)(huo)性(xing)(xing)部(bu)位直(zhi)接相關。 蛋白質(zhi)簇的(de)(de)(de)(de)(de)形(xing)成(cheng)(cheng)（通(tong)(tong)過FM觀(guan)(guan)察(cha)）以(yi)及酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)存(cun)在(zai)(zai)引(yin)起的(de)(de)(de)(de)(de)脂(zhi)(zhi)(zhi)(zhi)質(zhi)結(jie)(jie)構(gou)域分(fen)(fen)離似乎(hu)是主(zhu)(zhu)要(yao)(yao)在(zai)(zai)高(gao)表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)壓(ya)力(li)(li)(li)下(xia)調節(jie)酶(mei)(mei)(mei)(mei)(mei)活(huo)(huo)性(xing)(xing)的(de)(de)(de)(de)(de)重要(yao)(yao)因素(su)。 考慮(lv)到體(ti)內酶(mei)(mei)(mei)(mei)(mei)催(cui)(cui)化(hua)(hua)作用，其(qi)中(zhong)(zhong)(zhong)表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)堆積(ji)(ji)(ji)不會像(xiang)本(ben)(ben)(ben)研(yan)究中(zhong)(zhong)(zhong)所(suo)(suo)獲得的(de)(de)(de)(de)(de)那樣通(tong)(tong)過機械壓(ya)縮(suo)膜的(de)(de)(de)(de)(de)方(fang)(fang)式(shi)人為發生(sheng)，本(ben)(ben)(ben)文所(suo)(suo)述的(de)(de)(de)(de)(de)結(jie)(jie)果(guo)表(biao)(biao)(biao)(biao)(biao)(biao)明(ming)生(sheng)物(wu)膜中(zhong)(zhong)(zhong)可(ke)(ke)能存(cun)在(zai)(zai)信息(xi)傳遞機制。 這(zhe)種(zhong)轉導可(ke)(ke)以(yi)通(tong)(tong)過側(ce)膜組(zu)織(zhi)調節(jie)可(ke)(ke)溶性(xing)(xing)底(di)(di)物(wu)上GPI錨(mao)定(ding)(ding)酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)活(huo)(huo)性(xing)(xing)來(lai)(lai)實現，這(zhe)是特(te)定(ding)(ding)脂(zhi)(zhi)(zhi)(zhi)質(zhi)和(he)磷脂(zhi)(zhi)(zhi)(zhi)組(zu)成(cheng)(cheng)的(de)(de)(de)(de)(de)結(jie)(jie)果(guo)。 在(zai)(zai)高(gao)壓(ya)縮(suo)性(xing)(xing)條件下(xia)，影響(xiang)酶(mei)(mei)(mei)(mei)(mei)活(huo)(huo)性(xing)(xing)的(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)(yao)因素(su)是表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)密(mi)度，而最大(da)值來(lai)(lai)自表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)密(mi)度和(he)最佳(jia)(jia)膜彈性(xing)(xing)的(de)(de)(de)(de)(de)組(zu)合(he)，取(qu)決(jue)于脂(zhi)(zhi)(zhi)(zhi)質(zhi)/DSAP表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)填(tian)充(chong)。在(zai)(zai)高(gao)表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)壓(ya)力(li)(li)(li)下(xia)，導致多肽部(bu)分(fen)(fen)聚集和(he)界面(mian)(mian)(mian)(mian)(mian)(mian)流(liu)動(dong)性(xing)(xing)降(jiang)低（如壓(ya)縮(suo)模(mo)量顯著增(zeng)加所(suo)(suo)示(shi)(shi)）的(de)(de)(de)(de)(de)分(fen)(fen)子(zi)間相互(hu)(hu)作用可(ke)(ke)能與(yu)阻礙水溶性(xing)(xing)底(di)(di)物(wu)進入(ru)酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)催(cui)(cui)化(hua)(hua)活(huo)(huo)性(xing)(xing)部(bu)位直(zhi)接相關。蛋白質(zhi)簇的(de)(de)(de)(de)(de)形(xing)成(cheng)(cheng)（通(tong)(tong)過FM觀(guan)(guan)察(cha)）以(yi)及酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)存(cun)在(zai)(zai)引(yin)起的(de)(de)(de)(de)(de)脂(zhi)(zhi)(zhi)(zhi)質(zhi)結(jie)(jie)構(gou)域分(fen)(fen)離似乎(hu)是主(zhu)(zhu)要(yao)(yao)在(zai)(zai)高(gao)表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)壓(ya)力(li)(li)(li)下(xia)調節(jie)酶(mei)(mei)(mei)(mei)(mei)活(huo)(huo)性(xing)(xing)的(de)(de)(de)(de)(de)重要(yao)(yao)因素(su)。考慮(lv)到體(ti)內酶(mei)(mei)(mei)(mei)(mei)催(cui)(cui)化(hua)(hua)作用，其(qi)中(zhong)(zhong)(zhong)表(biao)(biao)(biao)(biao)(biao)(biao)面(mian)(mian)(mian)(mian)(mian)(mian)堆積(ji)(ji)(ji)不會像(xiang)本(ben)(ben)(ben)研(yan)究中(zhong)(zhong)(zhong)所(suo)(suo)獲得的(de)(de)(de)(de)(de)那樣通(tong)(tong)過機械壓(ya)縮(suo)膜的(de)(de)(de)(de)(de)方(fang)(fang)式(shi)人為發生(sheng)，本(ben)(ben)(ben)文所(suo)(suo)述的(de)(de)(de)(de)(de)結(jie)(jie)果(guo)表(biao)(biao)(biao)(biao)(biao)(biao)明(ming)生(sheng)物(wu)膜中(zhong)(zhong)(zhong)可(ke)(ke)能存(cun)在(zai)(zai)信息(xi)傳遞機制。這(zhe)種(zhong)轉導可(ke)(ke)以(yi)通(tong)(tong)過側(ce)膜組(zu)織(zhi)調節(jie)可(ke)(ke)溶性(xing)(xing)底(di)(di)物(wu)上GPI錨(mao)定(ding)(ding)酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)活(huo)(huo)性(xing)(xing)來(lai)(lai)實現，這(zhe)是特(te)定(ding)(ding)脂(zhi)(zhi)(zhi)(zhi)質(zhi)和(he)磷脂(zhi)(zhi)(zhi)(zhi)組(zu)成(cheng)(cheng)的(de)(de)(de)(de)(de)結(jie)(jie)果(guo)。

致謝

作者感謝FAPESP、CNPq、CAPES（巴(ba)西）、CONICET、FONCyT、SECyT和Fundacio’n Antorchas（阿根廷）的財政支持(chi)； 辛西婭·M·C·P·曼索(suo)博士感謝他仔細閱讀手稿。

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